Bone cancers are one of the tumors that are least taken into account in research, prioritizing other more common types of cancer such as: breast, lung, colon…etc. However, these can be just as lethal and originate in any bone structure of the body. The most common of these cancers is osteosarcoma, a primary malignant tumor that mainly affects children and adolescents. Furthermore, this type of tumor not only affects humans but to all types of vertebrates, although its diagnosis is facilitated with the use of an Xray machine, however the high maintenance cost of competent radiology equipment isolates its use only for hospitals or specialized veterinary centers in addition require prior training for the use of this equipment, this means that many research laboratories cannot
afford to acquire any of this equipment. In the present work we propose the use of diaphanization as a technique for: investigation, diagnosis and conservation of animal or human samples. Diaphanization is a technique for transparency of soft tissues and staining of bone tissue that is widely used in the study of the bone anatomy of a wide variety of vertebrates. In order to critically determine the effectiveness of this technique, we have carried out the diaphanization of different species of vertebrates based on two methodologies: double staining applied to mammals, birds, reptiles and amphibians, and simple “alizarin red” staining applied to fish. Finally, the clear material is obtained that can be observed with the naked eye and macroscopic and microscopic reviews can be performed if adequate microscopes are available.
You can see the project and download it here: Link to Drive
About Jorge José Domínguez Merina
I'm a biology student with solid practical training as a laboratory technician. I also have three years of experience caring for animals of various species. I have a great capacity for teamwork and a strong motivation for scientific research. My goal is to apply my skills and gain experience for my personal development.